UNIT-I
Nuclear Magnetic Resonance spectroscopy
Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical
shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and
applications
Mass Spectrometry
Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of
flight and Quadrupole, instrumentation, applications
UNIT-II
Thermal Methods of Analysis:
Principles, instrumentation and applications
of Thermo gravimetric Analysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)
X-Ray Diffraction Methods:
Origin of X-rays, basic aspects of crystals, X-
ray
Crystallography, rotating crystal technique, single crystal diffraction,powder
diffraction, structural elucidation and applications.
UNIT-III
Calibration and validation-as per ICH and USFDA guidelines
Calibration of following Instruments
- Electronic balance,
- UV-Visible spectrophotometer,
- IR spectrophotometer,
- Fluorimeter,
- Flame Photometer,
- HPLC
- GC
UNIT-IV
Radio immune assay:
Importance, various components, Principle, different
methods, Limitation and Applications of Radio immuno assay
Extraction techniques:
General principle and procedure involved in the solid
phase extraction and liquid-liquid extraction
UNIT-V
Hyphenated techniques
- LC-MS/MS
- GC-MS/MS
- HPTLC-MS
Recommended Books (Latest Editions)
1. Instrumental Methods of Chemical Analysis by B.K Sharma
2. Organic spectroscopy by Y.R Sharma
3. Text book of Pharmaceutical Analysis by Kenneth A. Connors
4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
6. Organic Chemistry by I. L. Finar
7. Organic spectroscopy by William Kemp
8. Quantitative Analysis of Drugs by D. C. Garrett
9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
10. Spectrophotometric identification of Organic Compounds by Silverstein
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