ADVANCED INSTRUMENTATION TECHNIQUES

ADVANCED INSTRUMENTATION TECHNIQUES
UNIT-I
 

Nuclear Magnetic Resonance spectroscopy 
Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications 

Mass Spectrometry
Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications 

UNIT-II 

Thermal Methods of Analysis: 
Principles, instrumentation and applications of Thermo gravimetric Analysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC) 

X-Ray Diffraction Methods: 
Origin of X-rays, basic aspects of crystals, X- ray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications. 

UNIT-III  

Calibration and validation-as per ICH and USFDA guidelines 
Calibration of following Instruments 
  • Electronic balance, 
  • UV-Visible spectrophotometer, 
  • IR spectrophotometer, 
  • Fluorimeter, 
  • Flame Photometer, 
  • HPLC  
  • GC 

UNIT-IV 

Radio immune assay:
Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay

Extraction techniques:
General principle and procedure involved in the solid phase extraction and liquid-liquid extraction 

UNIT-V  

Hyphenated techniques
  • LC-MS/MS
  • GC-MS/MS
  • HPTLC-MS 

Recommended Books (Latest Editions) 

1. Instrumental Methods of Chemical Analysis by B.K Sharma 

2. Organic spectroscopy by Y.R Sharma 

3. Text book of Pharmaceutical Analysis by Kenneth A. Connors 

4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel 

5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake 

6. Organic Chemistry by I. L. Finar 

7. Organic spectroscopy by William Kemp 

8. Quantitative Analysis of Drugs by D. C. Garrett 

9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi 

10. Spectrophotometric identification of Organic Compounds by Silverstein

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